Flow and Mass cytometry data analysis

AltraBio offers comprehensive services for flow and mass cytometry data analysis from gating to cross-sample analysis. To maximise the value of your experimental results and to speed up your process, AltraBio’s offer covers the entire data analysis workflow from discovery and diagnostic studies to optimization of population identification processes.

Gating step:

  • Identification of cell populations (unsupervised automated gating), description of identified cell populations (e.g., statistics, hypothesis tests, advanced visualisation)

  • Automatic and standardised application of your manual gating strategy to the full data of your study thanks to a unique service allowing to free the experimenter from this tedious and time consuming process. This approach possesses numerous advantages:

    • Standardisation of the analysis step 
    • Win of time --> 10 min per fcs file and per computer, 24/7 production
    • Win of reliability (reproducibility & accuracy) --> Analyst expertise concentration in the learning files
    • Remove the subjectivity and the variability of manual gating
    • Usable for deep and complexe gating strategies
    • Automation of quality controls and outlier identification

Cross Sample Analyses:

  • Initial import of raw data & gating workspaces from manual or automated gating, Preprocessing (e.g., compensation, normalization), Data Quality Control
  • Predictive modeling, correlation identification, advanced visualisation, clustering
  • Reagent panel selection, gating strategy optimization

More information...